PILR-alpha (paired immunoglobulin-like type 2 receptor-alpha; also FDF03) is one of two members of a small family of immunoregulatory Ig-superfamily receptors (1, 2). It is a counterpart to PILR-Beta and it likely gave rise to PILR-Beta through gene duplication and rearrangement (1). The PILRs represent one of many pairs of Ig-like domain-containing receptors that participate in immune regulation. PILR-alpha and -Beta should not be confused with the similarly named PIRs (also paired immunoglobulin-like receptors ), or the functionally-related SIRP and ILT/LILR/CD85/LIR family of receptors (2). While PIRs, ILTs and SIRPs contain three to six Ig-like domains in their extracellular region, PILR-alpha and -Beta show only one Ig-like region in their extracellular domain (ECD) (1, 2). Mouse PILR-alpha is a monomeric, 271 amino acid (aa) type I transmembrane (TM) protein (3). It contains a 167 aa ECD, a 21 aa TM segment, and a long, 83 aa cytoplasmic region. The ECD shows one V-type Ig-like domain between aa 39-157, while the cytoplasmic region contains two ITIMs (immunoreceptor Tyr-based inhibitory motifs) between aa’s 265-270 and 294-299. Given that ITIMs are known to interact with phosphatases such as PTPN6 and PTPN11, the presence of these motifs makes mouse PILR-alpha an inhibitory receptor. In human, activation of PILR-alpha inhibits CD32/FcgammaRII-induced calcium mobilization (3). Although CD99 is a known ligand for both PILR-alpha and -Beta (4), highest affinity binding seems to occur between CD99 and PILR-alpha (4). Mouse PILR-alpha is found on neutrophils and macrophages (4). Mouse PILR-alpha ECD is 43% and 69% aa identical to human and rat PILR-alpha ECD, respectively; it is 75% aa identical to the ECD of mouse PILR-Beta (3). One Potential isoform of PILR-alpha has been reported. It varies only within the first 28 aa of the signal sequence (5).
Source: A DNA sequence encoding the extracellular domain of mouse PILR-alpha (Leu 21-Val 197; Accession # NP_705730) was fused to a 6x histidine tag at the C-terminus. The protein was expressed in a mouse myeloma cell line, NS0.
Definition: Measured by its ability to bind rmCD99/mFc.
Applications:Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:Western Blot: 20.5kD migrates as an ~ 40-50kD protein in SDS-PAGE under reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant Mouse
Grade：Highly Purified
Purity：≥ 95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin ≤ 1EU.
Form：Supplied as a lyophilized powder in PBS. Reconstitute with sterile PBS to ≥ 0.1mg/ml.
