Noelin-1 (neuronal olfactomedin-related endoplasmic reticulum-localized-1), also known as olfactomedin-1 or pancortin, is a 75kD￥ secreted extracellular matrix glycoprotein expressed in the brain.1-3 Alternate promoter usage and splicing creates four forms of Noelin (Noelin 1-4) that are combinations of a common central region with alternate N and C termini.3, 4 Noelin-1 is the longest of the four isoforms, encoding a 16 amino acid (aa) signal sequence and a 469 aa mature protein with a coiled coil region for multimerization and an olfactomedin-like domain. The shortest isoforms, Noelin-3 and -4, are truncated within the coiled-coil region and lack the olfactomedin-like domain.4 In the species tested, these isoforms appear to oppose actions of the longer isoforms.5 All isoforms are capable of forming homo- or heterodimers through cysteines in their common central region.2 The C-terminus of Noelin-1 (mouse aa 482-485) contains a putative endoplasmic reticulum retention sequence that has not been included in the protein.6 Mouse Noelin-1 (aa 17-481) shares > 99%, 99%, 97%, 96% and 93% aa identity with corresponding regions of rat, human, opossum, chicken and Xenopus Noelin-1, respectively. In vivo expression patterns differ somewhat between species.1 In chick early development, Noelin-1 shows highest concentration in areas that produce neural crest cells and is produced by migrating neural crest cells.7 Overproduction of Noelin-1 prolongs neural crest cell formation.7 Noelin-1 is also expressed in mouse neural crest, but not frog.1-3 All three species express Noelin-1 later in differentiating neural tissues in the spinal cord, brain and cranial ganglia.1-3
Recombinant protein corresponding to Ser2-Arg481 from mouse Noelin-1, fused with a 6X His-Tag at the C-terminus, expressed in a mouse myeloma cell line, NS0.
Molecular Mass:Calculated molecular mass of ~55.7kD. As a result of glycosylation, the recombinant mouse Noelin-1 migrates as an ~80-90kD protein in SDS-PAGE under reducing conditions.
Activity:Measured by its ability to induce epithelial to mesenchymal transformation of MDCKII cells. Treatment of MDCKII cells with 200ng/ml of recombinant mouse Noelin-1 for 3 days is sufficient to induce the transformation of MDCKII cells.
Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, NSO cells
Grade：Purified
Purity：≥ 90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.Endotoxin: ≤ 0.1EU/1ug.
Form：Supplied as a lyophilized powder from PBS, pH 6.5. Reconstitute with 500ul sterile PBS.
