Neurexin 1, also known as NRXN1A, is a neuronal type I transmembrane molecule that is encoded by one of three Neurexin genes (1, 2). Mature rat Neurexin 1 is a 130-200kD N- and O-glycosylated protein with a 1424 amino acid (aa) extracellular domain (ECD) and a 55 aa cytoplasmic region. The ECD contains six LNS domains interspersed with three EGF-like domains and a juxtamembrane O-glycosylation region; the cytoplasmic domain contains a PDZ recognition motif (1, 2). The ECD of rat Neurexin 1 shares 92% and 97% aa sequence identity with human and mouse Neurexin 1 , respectively. It shares 68% aa sequence identity with rat Neurexin 2 and 3 . Transcription of the Neurexin 1 gene from an internal promoter gives rise to Neurexin 1 which lacks the first five LNS domains and all of the EGF-like domains (3). Neurexins are combinatorially spliced at five canonical sites, giving rise to over 1000 potential isoforms (3, 4). This recombinant protein corresponds to the ECD of SwissProt #Q63372, isoform 13, which has short deletions at splice sites 1 and 2. Neurexin 1 is primarily expressed in the brain where it is localized to presynaptic terminals (3, 5). It binds black widow spider toxin -latrotoxin, plus dystroglycan, neurexophilin 1 and 3, and the postsynaptic neuroligins (6-9). The interaction between Neurexins and neuroligins is complex and is restricted to particular combinations of splice forms of each binding partner (9). These interactions promote the differentiation and maintenance of postsynaptic GABA and NMDA, but not AMPA terminals (10, 11). At presynaptic terminals, as well as in pituitary melanotrophs, alpha Neurexins are required for coupling voltage-dependent calcium channel signaling to neurotransmitter or hormone release (12-14).
Recombinant protein corresponding to Leu31-Thr1431 (His1137Tyr) from rat Neurexin 1 alpha, fused to a C-terminal 6X His-Tag, expressed in NS0 cells.
N-terminal Sequence Analysis:Leu31
SDS-PAGE:165-175kD, reducing conditions.
Biological Activity:Measured by its binding ability in a functional ELISA. Immobilized rrNRXN-1a at 0.5ug/ml (100ul/well) can bind biotinylated rrNXPH-1 with a linear range of 0.03-2ug/ml.
Storage and Stability:Lyophilized and reconstituted products may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, NS0 cells
Grade：Purified
Purity：≥80%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: ≤0.1EU/ug (LAL).
Form：Supplied as a lyophilized powder in PBS. Reconstitute with 500ul sterile PBS.
