DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65kD type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (1). Mature mouse DNAM has a 236 amino acid (aa) extracellular domain (ECD) that contains two Ig-like C2-set domains, and possesses a 58 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins (1, 2). Within the ECD, mouse DNAM-1 shares 52% and 86% aa sequence identity with human and rat DNAM-1, respectively. Additional cDNA transcripts of mouse DNAM-1 may give rise to secreted or transmembrane isoforms with ECD deletions. DNAM-1 is expressed on several lymphoid and myeloid cell types and interacts with CD155/PVR and Nectin-2/CD112 (2-4). Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells (3-7), induces dendritic cell maturation, initiates megakaryocyte and activated platelet adhesion to vascular endothelial cells, and stimulates monocyte extravasation; conversely, it inhibits the formation of osteoclasts (8-11). Platelet-endothelium interactions that are mediated by DNAM-1 enable the metastasis of tumor cells to the lung (12). On activated, but not resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to tyrosine and serine phosphorylation of DNAM-1 during activation (7, 10, 13-15).
Source: A DNA sequence encoding the extracellular domain of mouse DNAM-1 (Met 1-Pro 254; Accession # Q8K4F0) was fused to the Fc region of mouse IgG2A via a polypeptide linker. The chimeric protein was expressed in a mouse myeloma cell line, NS0.
Definition: Measured by its ability to bind biotinylated rmNectin-2.
Applications:Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:Western Blot: 53.9kD protein migrates as an ~ 70-90kD protein in SDS-PAGE under reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant Mouse
Grade：Purified
Purity：≥ 80%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin ≤ 1EU.
Form：Supplied as a lyophilized powder in PBS. Reconstitute with sterile PBS to ≥ 0.1mg/ml.
