CD2-like receptor activating cytotoxic cells (CRACC), also known as CS1, novel Ly9, SLAMF7, and CD319, is a 66kD type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family (1). Mature mouse CRACC consists of a 202 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set domain and one Ig-like C2-set domain, a 21 aa transmembrane segment, and an 88 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs ITSMs (2, 3). Within the ECD, mouse CRACC shares 53% aa sequence identity with human CRACC. It shares 19%-35% aa sequence identity with comparable regions of other mouse SLAM proteins including 2B4, BLAME, CD2F-10, CD84, CD229, NTB-A, and SLAM/CD150. Additional isoforms of mouse CRACC are distinguished by deletions and/or substitutions in their cytoplasmic domains. CRACC is expressed on the surface of NK cells, CD8+ T cells, activated B cells, and mature dendritic cells (4, 5). It interacts homophilically to induce NK, CTL, and B cell activation (4-7). In human NK cells, activated CRACC transmits signals following association with the adaptor protein EAT-2 (8).
A DNA sequence encoding the extracellular domain of mouse CRACC (Ala 22-Gly 224; Accession # Q8BHK6) was fused to a 10X histidine tag at the C-terminus. The protein was expressed in Chinese Hamster Ovary cells.
Calculated molecular mass of 23.6kD. As a result of glycosylation, the recombinant protein migrates as an ~ 34-55kD protein in SDS-PAGE under reducing conditions.
Activity: Measured by its ability to bind biotinylated rmCRACC.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, CHO cells
Grade：Highly Purified
Purity：≥95% (SDS-PAGE under reducing conditions and visualized by silver stain) Endotoxin: ≤1EU/1ug
Form：Supplied as a lyophilized powder in PBS. Reconstitute with 500ul sterile PBS.
