Collectins constitute a family of C-type lectins that recognize molecular patterns expressed on pathogens. Members of this glycoprotein family contain a N-terminal domain, a collagen-like domain, a neck region, and a C-terminal carbohydrate recognition domain (CRD). Collectins are typically secreted molecules, although CL-P1 is membrane bound and CL-L1 is found in the cytoplasm. Collectin kidney 1 (CL-K1), also known as collectin subfamily member 11 (COLEC11), is a 37kD collectin that circulates in the serum. It associates into disulfide-linked oligomers and preferentially interacts with fucose residues in a calcium-dependent manner. Mature mouse CL-K1 shares 94% and 98% amino acid sequence identity with human and rat CL-K1, respectively. Within the CRD, mouse CL-K1 shares 52% aa sequence identity with CL-L1 and 23%-32% aa sequence identity with collectins CL-P1, MBL, SP-A, and SP-D.
DNA sequence encoding mouse CL-K1 (Gln27-Leu272), fused to a 6X His-Tag at the C-terminus, expressed in a mouse myeloma cell line, NS0.
Activity:Measured by its ability to bind Alpha-L-Fucose.
Applications:Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions:Western Blot: As a result of glycosylation the recombinant mouse CL-K1 migrates as an ~33-38kD protein in SDS-PAGE under reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, NS0
Grade：Purified
Purity：≥85%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. 33-38kD Endotoxin: ≤0.1EU/1ug (LAL)
Form：Supplied as a lyophilized powder from PBS. Reconstitute with 500ul sterile PBS.
