CD200 R1, also known as OX-2 receptor, is a 90kD, type I transmembrane protein that belongs to the immunoglobulin superfamily. CD200 R1 is important in the regulation of myeloid cell activity.1-3 The mouse CD200 R1 cDNA encodes a 326 aa precursor that includes a 25 aa signal sequence, a 213 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 67 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain.4 Within the ECD, mouse CD200 R1 shares 56% and 70% aa sequence identity with human and rat CD200 R1, respectively. The ECD of mouse CD200 R1 shares 69%, 38%, 79%, and 83% aa sequence identity with the ECD of CD200 R2, 3, 4, and a CD200 R-like molecule, respectively. CD200 R1 is expressed primarily on mast cells, basophils, macrophages, and dendritic cells, 5-7 while its ligand, CD200, is widely distributed.8 Disruption of this receptor-ligand pair by knockout of the CD200 gene leads to increased macrophage number and activation, plus a predisposition to autoimmune disorders.9 Association of CD200 with CD200 R1 takes place between their respective N-terminal Ig-like domains.10 The CD200 R-like molecules may interact differently with CD200.11, 12 The cytoplasmic domain of CD200 R1 contains two non-ITIM tyrosine residues which are required for propagating its inhibitory signals.13-15 CD200 R-like molecules, in contrast, are potentially activating receptors by means of their association with DAP12.4, 16
A DNA sequence encoding the extracellular domain of mouse CD200 R1 (Thr26-Pro 238; Accession # BAE42266) was fused to the carboxy-terminal Fc region of human IgG1 (Pro100-Lys330) via a linker peptide. The chimeric protein was expressed in a mouse myeloma cell line, NS0
Calculated molecular mass of 49.7kD. As a result of glycosylation, the recombinant monomer migrates as an ~ 90-100kD protein in SDS-PAGE under reducing conditions.
Activity:Measured by its ability to bind in a functional ELISA.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, NS0 cells
Grade：Purified
Purity：≥85%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: ≤1EU/1ug.
Form：Supplied as a lyophilized powder in PBS. Reconstitute with 500ul sterile PBS.
