CD157, also known as bone marrow stromal cell antigen 1 (BST-1), is a glycosyl phosphatidylinositol anchored membrane protein that belongs to the CD38 family (1). CD157 was discovered in a bone marrow stromal cell line where it facilitates pre-B-cell growth (2, 3). Along with CD38, CD157 is a bifunctional ectoenzyme that exhibits both ADP-ribosyl cyclase and cyclic ADP ribose hydrolase activities (2). It may play a role in rheumatoid arthritis (RA) due to its enhanced expression in RA-derived bone marrow stromal cell lines (3). CD157 has been predicted to function as a cell surface receptor and an immunoregulatory molecule (4).
Source: Recombinant human CD157 encoded by the BST-1 gene (rhCD157; aa29 to 292; GARARWRGEGT. Accession # Q10588) was expressed with a C-terminal 6X His tag in a murine myeloma cell line, NS0.
Definition: Measured by its ability to cyclize nicotinamide guanine dinucleotide (NGD+).
Specific Activity: ≥ 20pmol/min/ug.
Applications:Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:Western Blot: Predicted 31kD and migrates as a doublet at 38 and 40kD bands in SDS-PAGE under reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant human
Grade：Highly Purified
Purity：≥ 95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin ≤ 1EU.
Form：Supplied as a 0.2um filtered liquid in 25mM Tris, 0.15M sodium chloride, pH 7.5.
