ARSG is a member of the aryl sulfatase family of enzymes with a high degree of amino acid sequence similarity to ARSA and ARSB. ARSG mRNA is expressed in a ubiquitous manner, being detected in most adult and embryonic tissues of the mouse (1).
Recombinant mouse Arylsulfatase G encoded by the Arsg gene (rmARSG; amino acid residues 17-525; Accession # NP_082986) (1) was expressed with an N-terminal signal sequence and a C-terminal 10X His tag in a Chinese Hamster Ovary cell line.
Calculated molecular mass of 55kD and migrates as a 6 kD band in SDS-PAGE under reducing conditions.
Activity: Measured by its ability to hydrolyze the substrate 4-nitrocatechol sulfate (PNCS).
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant Mouse
Grade：Highly Purified
Purity：≥ 95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.Endotoxin: ≤ 1EU/1ug.
Form：Supplied as a liquid in 25mM Tris, 0.15M sodium chlroide, pH 7.5.
