Fatty Acid Synthase (FASN) catalyzes the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminal of FASN is fused in-frame with the C-terminal of ER-alpha. FASN is highly expressed in adipocytes and liver. FASN is a central enzyme in de novo lipogenesis. FASN is a target for SREBP and is upregulated by LXR activation; it is also one of the accepted markers for insulin resistance, SREBP and LXR activation. FASN: Bovine: 2513aa; Pig: 2411aa; Chick: 2511aa; Mouse: 2504aa; Rat: 2505aa; Human: 2511aa; 273.4kD; 17q25. Expression: Ubiquitous; more prominent expression in brain, lungs, and liver.
Source:Synthetic peptide corresponding to 18aa of ~N-termial of human FASN (KLH) (P49327). Applications:Suitable for use in ELISA and Antibody Blocking. Other applications not tested.
Recommended Dilution:ELISA: 50-100ng
Antibody Blocking: 5-10ug/1ug of Affinity Purified antibody or 1ul of serum
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Human synthetic peptide
Grade：Purified
Purity：Purified.
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes human FASN. Species Crossreactivity: mouse, rat bovine-100%; pig-94%; and chick 83%.
