Corresponding antibody Recognizes human Beta-Actin. Species Crossreactivity: Mouse, rat, human, chicken, frog, etc.
Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis. Actin is one of the most conserved cellular protein. Atleast 6 actin isoforms have been identified by protein sequence analyses. Four actin isoforms represent the differentiation markers of muscle tissues. There are three a-actins: a-skeletal, a-cardiac, and a-smooth muscle), one b-actin (b-non-muscle), and two g-actins (g-smooth muscle and g-non-muscle). Actin isoform are >90% conserved, except in the N-terminal 18-aa (50-60% homology). Beta-actin protein and mRNA levels are often used as a reference for comparing changes in cellular protein/mRNA levels by Western or Northern blots.
Source:Synthetic peptide, 15-aa corresponding to human Beta-Actin (KLH).
Applications:Suitable for use in ELISA and Antibody Blocking. Other applications not tested.
Recommended Dilution:ELISA:Antibody Blocking:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
