The Mouse IgG ELISA Kit is an in vitro immunoassay for the quantification of IgG circulating in serum or in other appropriately qualified samples from tissue fluids (e.g., saliva, mucosa), or in cultures of mouse cells.
Specificity:The antibodies used in this kit have been shown by immunoelectrophoresis and ELISA to react specifically with IgG, and have essentially no reactivity with IgM, IgA, IgE or any other mouse serum proteins.
Precision:IgG concentrations were measured with very good within-assay (4.4 to 7.3 %CV) and good between-assay (9.7 to 11.0 %CV) reproducibility.
Kit Components:I1904-15A Microtiter Plate 1x96 wellsI1904-15B Sample Diluent Concentrate 20X 1x10mlI1904-15C Wash Solution Concentrate 100X 1x10 ml I1904-15D IgG (HRP) 100X 1x150ulI1904-15E Standard 10ng/ml 1x650ulI1904-15F Standard 25ng/ml 1x650ulI1904-15G Standard 50ng/ml 1x650ulI1904-15H Standard 100ng/ml 1x650ulI1904-15J Standard 200ng/ml 1x650ulI1904-15K Positive Control 1x650ulI1904-15L TMB Substrate 1x12ml I1904-15M Stop Solution 1x12 ml
Storage and Stability:May be stored at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
