85-1540-12　PRMT1, isoform2, Recombinant, Mouse, 1-353 aa, mouse, His-MBP tag (Protein arginine N Methyltransferase 1) 100ug　P9004-01B

特徴

• Murine PRMT1 isoform2, 1-353 aa, His-MBP tagged, Recombinant, E. coli
• Full name: protein arginine N-methyltransferase 1
• NCBI Accession #: AAF37293
• Synonyms: Mrmt1, Hrmt112, AW214366, Protein arginine N-methytransferase 1
• Protein arginine N-methyltransferase 1(PRMT1) is a type I methyltransferase that transfers a methyl group from S-adenosylmethionine to guanidino nitrogens of arginine residues to form monomethylarginine and asymmetric dimethylarginine. Functions of type I arginine methylation in proteins may include regulation of transcription, modulation of the affinity of nucleic acid-binding proteins, regulation of interferon signaling pathways, and targeting of nuclear proteins.
• Mouse PRMT1(AAF37293) shares 99.9% sequence identity with the human proteins (NP_938074).
• Recombinant mouse PRMT1, fused to His-MBP tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
• Molecular Weight: 84kD (750 aa)
• Specific Activity: 10,000unit/ml
• Unit Definition: One unit will transfer 1pmol of methyl group to synthetic peptide of histone H4 for 10 minutes at 37℃.
• Sequence: MHHHHHHMKI EEGKLVIWIN GDKGYNGLAE VGKKFEKDTG IKVTVEHPDK LEEKFPQVAA TGDGPDIIFW AHDRFGGYAQ SGLLAEITPD KAFQDKLYPF TWDAVRYNGK LIAYPIAVEA LSLIYNKDLL PNPPKTWEEI PALDKELKAK GKSALMFNLQ EPYFTWPLIA ADGGYAFKYE NGKYDIKDVG VDNAGAKAGL TFLVDLIKNK HMNADTDYSI AEAAFNKGET AMTINGPWAW SNIDTSKVNY GVTVLPTFKG QPSKPFVGVL SAGINAASPN KELAKEFLEN YLLTDEGLEA VNKDKPLGAV ALKSYEEELA KDPRIAATME NAQKGEIMPN IPQMSAFWYA VRTAVINAAS GRQTVDEALK DAQTNSSSNN NNNNNNNNLG IEGRGSHMAA AEAANCIMEV SCGQAESSEK PNAEDMTSKD YYFDSYAHFG IHEEMLKDEV RTLTYRNSMF HNRHLFKDKV VLDVGSGTGI LCMFAAKAGA RKVIGIECSS ISDYAVKIVK ANKLDHVVTI IKGKVEEVEL PVEKVDIIIS EWMGYCLFYE SMLNTVLHAR DKWLAPDGLI FPDRATLYVT AIEDRQYKDY KIHWWENVYG FDMSCIKDVA IKEPLVDVVD PKQLVTNACL IKEVDIYTVK VEDLTFTSPF CLQVKRNDYV HALVAYFNIE FTRCHKRTGF STSPESPYTH WKQTVFYMED YLTVKTGEEI FGTIGMRPNA KNNRDLDFTI DLDFKGQLCE LSCSTDYRMR
• Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.