Multiple Tag is a specially designed fusion protein for use as a positive control for Western blot or Dot blot. The fusion protein is a 40kD protein containing 16 commonly used tags from N-terminus to C-terminus in the order as shown in the table:TagSequenceTrx MSDKIIHLTD DSFDTDVLKA DGAILVDFWA EWCGPCKMIA PILDEIADEY QGKLTVAKLN IDQNPGTAPK YGIRGIPTLL LFKNGEVAAT KVGALSKGQL KEFLDANLAT7CMASMTGGQQMGHSVQPELAPEDPEDCc-MycEQKLISEEDLCVSV-gYTDIEMNRLGKGlu-GluCEEEEYMPMEV5GKPIPNPLLGLDSTE-tagGAPVPYPDPLEPRFlag-tagDYKDDDDKS-tagKETAAAKFERQHMDSHAYPYDVPDYACKT3KPPTPPPEPETE2GVSSTSSDFRDRAU1DTYRYIAU5TDFYLK6x HisHHHHHH
Applications:Suitable for use in Western Blot and Dot Blot. Other applications not tested.
Recommended Dilution:Western Blot: 0.04-0.2ul per lane of minigel. For accurate loading, dilute with PBS
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store lyophilized powder at -20°C or lower. Reconstitute by adding sterile dH2O. Aliquot reconstituted product to avoid repeated freezing and thawing, and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：200ug
Source Antigen：E. coli
Grade：Purified
Purity：>95% by SDS-PAGE and Coomassie blute staining. Purified from E. coli cell lysate.
Form：Supplied as a lyophilized powder. Reconstitute with 200ul of ddH2O.
