Human uPAR. The urokinase-type plasminogen activator (uPA) is one of two activators that converts the extracellular zymogen plasminogen to plasmin, a serine protease that is involved in a variety of normal and pathological processes that require cell migration and/or tissue destruction. uPA is synthesized and released from cells as a single-chain (sc) pro-enzyme with limited enzymatic activity and is converted to an active two-chain (tc) disulfide-linked active enzyme by plasmin and other specific proteinases. Both the scuPA and tcuPA bind with high-affinity to the cell surface via the glycosyl phosphatidylinositol-linked receptor uPAR which serves to localize the uPA proteolytic activity. The enzymatic activity of scuPA has also been shown to be enhanced by binding to uPAR. Independent of their proteolytic activity, the uPA/uPAR interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can interact with integrins to suppress normal integrin adhesive function and promote adhesion to vitronectin through a high affinity vitronectin binding site on uPAR. uPAR cDNA encodes a 335 amino acid residue precursor protein with a 22 aa residue signal peptide, five potential N-linked glycosylation sites and a C-terminal GPI-anchor site. An alternate spliced variant of uPAR encoding a secreted soluble form of uPAR also exists. Human and mouse uPAR share approximately 60% amino acid sequence identity and the receptor-ligand interaction is strictly species-specific.
Source:Recombinant protein corresponding to Leu23-Arg303 with a C-Terminal His-Tag from human uPAR, expressed in NS0 cells. Swiss/UniProt Accession: Q03405
Applications:Suitable for use in ELISA. Other applications have not been tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Biological Activity:Measured by its ability in functional ELISA. Immobilized recombinant uPAR at 5ug/ml (100ul/well) can bind recombinant human uPA with a linear range of 0.3-20ng/ml.
Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS, 0.1% BSA or HSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：NS0-derived recombinant human uPAR. Synthetic peptide corresponding to a region between aa1 and 303.
Grade：Highly Purified
Purity：≥95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: ≤1EU/ug (LAL)
Form：Supplied as a lyophilized powder from PBS, containing 50ug of BSA and 1ug carrier protein. Reconstitute with 10ml PBS to a concentration of 10ug/ml.
