Ficolin-1 (fibrinogen/collagen-like), also called M-ficolin, is a member of the ficolin family of secreted pattern recognition proteins in the lectin complement activation pathway (1-3). Ficolin-1 is expressed by monocytes, neutrophils and type II alveolar epithelial cells (2-5). It is proposed to be a locally-acting lectin released in a regulated manner (4). Ficolin-1 is not found in plasma, but is detected on the surface of circulating monocytes (4-6). The 35kD, 326 amino acid (aa) human ficolin-1 contains a 28 aa signal sequence, an N-terminal collagen domain and a C-terminal fibrinogen-like (FBG) domain that includes a calcium binding site and one potential N-glycosylation site. Both the collagen and FBG domains mediate trimer formation (7). Like ficolin-2, larger homo-multimers of ficolin-1 exist and are likely formed by disulfide bonds at the N-terminus. Sizes corresponding to 12 or 18 subunit oligomers are reported (4, 6, 7). The FBG domain of Ficolin-1 binds microbial ligands that contain acetylated compounds (6). Ligands identified include N-acetyl glucosamine, N-acetyl galactosamine and sialyl-N-acetyllactosamine (4-7). Like other ficolins, ficolin-1 associates with, and activates the MBL-associated serine protease (MASP) complex, which activates the complement pathway by cleaving C4, contributing to the innate immune response (4-6). The ficolin-1 gene is polymorphic, showing at least ten single nucleotide polymorphisms in the promoter and coding regions (1).
Product Description:Mouse myeloma NSO cell line-derived recombinant human Ficolin-1 (Gln28 thru Ala326, NCBI accession #O00602) with a C-terminal 10x His tag.
Activity:Measured by its ability to bind immobilized Fetuin in a functional ELISA (Liu, Y. et al. (2005) J. Immunol. 175:3150).
Storage and Stability:Lyophilized powder is stable for 12 months at -20°C Reconstituted product is stable for 3 months at -20°C or lower under sterile conditions. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Source Antigen：Recombinant, Human CD33 Signal Peptide
Grade：Highly Purified
Purity：> 95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: < 1.0 EU per 1ug of the protein as determined by the LAL method.
Form：Supplied as a lyophilized powder in PBS. Reconstitute with sterile PBS to a concentration of 0.1mg/ml.
Calc Applications Abbrev：E
Immunogen：NS0-derived recombinant human Ficolin-1.
