Brevican, also called BEHAB, is a secreted member of the the lectican family of proteoglycans that share a common domain structure. Brevican contains an Ig-like V-set domain, two link domains, a Glu-rich region, a central region with glycosaminoglycan (GAG) modifications, an EGF-like domain, a C-type lectin domain, and a C-terminal Sushi/CRP-like domain. Brevican is restricted to the CNS and is expressed by astrocytes, oligodendrocytes, and neurons. A GPI-anchored alternate splice form exists that is truncated following the central (GAG) region. Brevican is cleaved by multiple proteases and exists in a number of distinct fragments. Full-length brevican consists of a 97kD core protein with up to approximately 100kD of attached chondroitin sulfate but not heparan sulfate chains. Brevican associates with the extracellular matrix, perineuronal nets, and astrocyte cell surfaces by means of its chondroitin sulfate, GPI anchor, hyaluronic acid-binding link domains, and the core protein. The secreted isoform is dominant during brain development and is upregulated in astrocytes following brain injury. In human and rat, an under-glycosylated form of brevican is upregulated in highly aggressive glioma but not in low-grade glioma or other brain pathologies. In mouse and rat, levels of an ADAMTS4-generated 55kD N-terminal fragment increase during remodeling after excitotoxic injury. Human brevican shares 90%, 80%, and 80% aa sequence identity with bovine, mouse, and rat brevican, respectively. Within the Iglike and two link domains, brevican shares 45% 51% aa sequence identity with aggrecan, neurocan, and versican
DNA sequence encoding the signal peptide from human CD33, Asp23-Pro911, fused to the C-terminal polyhistidine tagged mature form of human Brevican. The protein was expressed in a mouse myeloma cell line, NS0. The recombinant polyhistidine tagged human Brevican, generated by proteolytic removal of the signal peptide region, is a monomeric protein. Based on N-terminal amino acid sequencing, the mature recombinant human Brevican protein begins with Asp 23 and has a calculated molecular mass of 97.7kD. As a result of glycosylation, the recombinant protein migrates an approximately 130-170kD protein in SDS-PAGE under reducing conditions.
Activity:Measured by its ability to bind biotinylated hyaluronan in a functional ELISA type binding assay with an estimated Kd < 3nM.
Storage and Stability:Lyophilized and reconstituted products may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity：≥85%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: ≤1EU/1ug (LAL)
Form：Supplied as a lyophilized powder from PBS and 0.1mM EDTA, pH 6.8. Reconstitute with 250ul sterile ddH2O. For long term storage add a carrier protein (e.g. 0.1% HSA or BSA).
