Human BLAME (B-lymphocyte activator macrophage expressed), also known as SLAM family member 8, is a type I transmembrane protein that belongs to the CD2 subset of immunoglobulin superfamily cell receptors. CD2 family proteins function as adhesion molecules and modulators of immune responses. Mature human BLAME consists of a 211aa ECD that contains two Ig V-like domains, a 21aa transmembrane segment, and a 31aa cytoplasmic tail that lacks recognizable signaling motifs (3). Within the ECD, human BLAME shares 19-26% aa sequence identity with human 2B4, CD2, CD2F-10, CD48, CD58, CD84, CD229, CRACC, NTB-A, and SLAM. It shares 79% aa sequence identity with the ECD of mouse BLAME. BLAME is expressed on dendritic cells and IFN-γ stimulated monocytes. Overexpression of BLAME in bone marrow cells leads to an increase in the peritoneal B1b population of B lymphocytes.
Molecular Mass:The recombinant human BLAME is a monomeric protein. Based on N-terminal amino acid sequencing, the recombinant protein starts at Ala 23 and it has a calculated molecular mass of 24.7kD. As a result of glycosylation, the recombinant monomer migrates as an approximately 35-40kD protein in SDS-PAGE under reducing conditions.
Endotoxin:≤ 1.0 EU per 1ug of the protein as determined by the LAL method.
Biological Activity:Measured by its ability to bind biotinylated rhBLAME in a functional ELISA type binding assay.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
