Mouse Sphingosine kinase 1 (GenBank Accession No. AF415213), full length with N-terminal His tag, expressed in a Baculovirus infected Sf9 cell expression system.
Specific Activity:20 U/ug. Sphingosine kinase activity was determined using D-erythrosphingosine and ATP as substrates. A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1pmol of S1P/min.
Applications:Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
