85-1538-38　SIRT 3, Recombinant, Human, GST-Tag (Sirtuin 3, Sir2-related Protein Type 3, Sir2-like 3) 100ug　S1013-88F2

特徴

• Human Sirtuin 3 (GenBank Accession No. NM_012239), amino acids 102- 399 with N-terminal GST tag, expressed in E. coli expression system.
• Specific Activity:6 U/ug. One U =1 pmol/min
• Assay Condition:25mM Tris/Cl, pH 8.0, 137mM NaCl, 2.7mM KCl, 1mM MgCl2 and 0.1mg/ml BSA, 30uM BPS HDAC substrate, 200uM NAD+ and 20ng/ul Sirtuin 3.
• Incubation Condition: 30 min at 30ºC
• Application:Useful for the study of enzyme kinetics, screening inhibitors and selectivity profiling.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

仕様

• Size：100ug
• Source Antigen：Recombinant, E. coli
• Grade：Highly Purified
• Purity：≥ 90% by SDS-PAGE
• Form：Supplied as a liquid in 50mM Tris-HCl, pH 8.0, 138mM NaCl, 20mM glutathione and 10% glycerol.
• Molecular Weight Non Ab：59
• Swiss Prot Number：Q9NTG7
• EU Commodity Code：30021019
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