Complex of N-terminal GST tagged recombinant full-length human p110d ( GenBank Accession No. NM_005026), and recombinant full length, human p85a (GenBank Accession No. XM_043865). Coexpressed in a Baculovirus infected Sf9 cell expression system.
Specific Activity:3nmol/mg/min using phosphatidylinositol as the substrate. p110d/p85a activity was determined using PIP2 and ATP as substrates.
Assay Conditions:Enzyme reaction was carried out for 15 minutes at 30ºC using 5-200ng of P4073-30B1 in a buffer containing 40mM Tris-HCl, pH 7.5, 20mM magnesium chloride, 100um ATP, 200uM L-alpha-phosphatidylinositol/L-alpha-phosphatidylserine (PI/PS) substrate.
Applications:Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
