Human PDE1A (GenBank Accession No. NM_005019), full length with N-terminal GST tag, expressed in a Baculovirus infected Sf9 cell expression system.
Specific Activity:123 U/ug; 1 unit will lease 1nmole phosphate per minute from 400uM 5'- AMP at 30°C in a reaction buffer of 10mM Tris-HCl, pH 7.4, 0.2mM MgCl2.
Applications:Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
仕様
Size：10ug
Source Antigen：Recombinant, Sf9 insect cells
Grade：Purified
Purity：≥ 30% by SDS-PAGE
Form：Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM NaCl, 0.05% Tween 20, 50% glycerol, 20mM glutathione and 3mM DTT.
