Recombinant full-length house PDK1 was expressed by baculovirus in Sf9 insect cells using a N-terminal His tag. The gene accession number is NM_002613.
Specific Activity:As Reported
Assay Conditions:Assay was done in Kinase buffer containing 0.2mM DTT using PDKtide substrate (0.1mg/ml) and 20uM ATP. Reaction was done at 30ºC fro 45 min. Amount of ATP transferred was measured using Kinase-Glo reagent.
Applications:Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
