Human HDAC5 (GenBank Accession No. NM_001015053), full length with N-terminal GST tag, expressed in baculovirus expression system.
Specific Activity:~1000pmol/min/ug.
Assay Condition:25mM Tris/Cl, pH 8.0, 137mM sodium chloride, 2.7mM KCl, 1mM MgCl2, and 0.1mg/ml BSA, 20uM H5109-66G substrate and 0.8-50ng/ul HDAC5. Incubate 30 min at 37ºC.
Incubation Condition:60 min at 30ºC.
Applications:Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Storage and Stability:Store at -70°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:5ug
Source Antigen:Recombinant, Baculovirus expression system
Grade:Purified
Purity:Purified
Form:Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM KCl, 1.6mM Glutathione, and 20% glycerol.