Assay Condition:The enzyme reaction was carried out at 37ºC for 20 min in a buffer containing 20mM Tris-HCl, pH 7.4, 10mM of MgCl2, 1mM of MnCl2, 1mM of DTT and 1mM of ATP, 1ug/ml poly(Glu4-Tyr) peptide substrate.
Applications:Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
仕様
Size:10ug
Source Antigen:Recombinant, Baculovirus infected Sf9 cell expression system
Grade:Highly Purified
Purity:≥ 90%
Form:Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% Tween 20, 3mM DTT, 50% glycerol.