Human EGFR T790M (GenBank Accession No. NM_005228), (aa668-1210) with N-terminal GST tag.
Applications:Useful for the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested.
Specific Activity:~0.9 pmol/min/ug
Recommended Dilution:Assay Conditions:50mM HEPES, pH 7.5, 10mM of MgCl2, 1mM of EDTA, 0.01% Brij-35, 200uM ATP. Substrate 2uM Tyr peptide 4 at RT for 1 hour
Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10ug
Source Antigen：Recombinant, Baculovirus expression system
Grade：Purified
Purity：~40%
Form：Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 138mM sodium chloride, 0.05% Tween 20, 3mM DTT, 50% glycerol.
