Human Akt1 (GenBank Accession No. NM_001014432), full length (a.a. 1-480) with N-terminal GST tag, co-expressed with PDK1 in an Baculovirus infected Sf9 cell expression system.
Specific Activity:~12pmol/min/ug
Assay Conditons:Enzyme reaction is conducted in a buffer containing 50mM HEPES, pH 7.5, 10mM magnesium chloride, 1mM EGTA, 200uM ATP, 0.01% Brij-35, and 2uM substrate (SER/THR 6) at RT for 1 hour.
Applications:Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
仕様
Size：10ug
Source Antigen：Recombinant, Sf9 insect cells
Grade：Highly Purified
Purity：≥70% (SDS-PAGE)
Form：Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% Tween 20, 3mM DTT, 50% glycerol.
