This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-13 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody specific for IL-13 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-13 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Kit Components:I8435-01A: Microtiter Plate, 1x96 wellsI8435-01B: Pab (HRP), 1x21mlI8435-01C: Standard*, 1x20ngI8435-01D: Assay Diluent: 1x11mlI8435-01E: (Cell Culture Supernatant), 1x21mlI8435-01F: Calibrator Diluent (Serum/Plasma), 1x21mlI8435-01G: Wash Buffer (25X), 1x21mlI8435-01H: Color Reagent A (H2O2), 1x12.5mlI8435-01J: Color Reagent B (TMB), 1x12.5mlI8435-01K: Stop Solution, 1x6ml
Storage and Stability:Store all components at 4°C. Stable for 6 months. *Store I8435-01C at -20°C after reconstitution. Components are stable for 1 month at the appropriate temperature after opening/reconstitution.
