BioAssay™ Tissue Set includes Genomic DNA, RNA, and Protein isolated from the same piece of biomaterials, such as tissues or cells. Set includes 50ug Total RNA 10ug Genomic DNA, and 100ug Protein. Set is particularly important for functional genomics and proteomics study, such as gene regulation, expression of genes and protein, among DNA, RNA, and protein.
Produced by proprietary techniques, this set exhibits the same high qualities as those products isolated separately by conventional methods. Compared to commercial kits and reagents for RNA isolation, which can produce varying quality of genomic DNA and protein, the RNA quality is typically inferior due to protein isolation methods, which can yield only 10-30%. This means many proteins may be missing, and is not suitable for protein expression profiling, protein quantitation and protein purification.
In contrast, the BioAssay™ Tissue Set is ready to use and contains highly purified DNA, RNA and Protein suitable for use in protein expression profiling, protein quantitation, and protein purification.
Quality Control:Genomic DNA:1. The quality and purity of genomic DNA were tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0 (detected in 10mM Tris-Cl, pH 7.5)
2. RNase treatment to ensure the RNA contamination is eliminated
3. Hind III digestion test: genomic DNA was successfully digested by Hind III
4. PCR test: A specific fragment of actin gene, about 800bp, was successfully amplified from the genomic DNA.
Total RNA:1. The integrity of the RNA is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA when electrophoreses on a denaturing agarose gel. The quality and purity of total RNA were tested by spectrophotometer. A260/280 is between 1.8 and 2.0 (detected in 10mM Tris-Cl, pH 7.5)
2. The RNA is treated by DNase I, and is tested as DNA free RNA by PCR
3. cDNA synthesis is successfully performed by using this RNA as template
Note:1. Total RNA from some rare tissues and tumor tissues may not be treated by DNase I2. To visualize the RNA images on agarose gel, we recommend using1% agarose gel in 1x MOPS buffer with formaldehyde. Degraded RNA images may result from using other gel systems, such as TAE gel, TBE gel, Urea gel, etc.
Total Protein:1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining, the pattern is consistent with each lot
2. The isolated protein is Western analyzed by either GAPDH or b-actin antibody, and the expression level is consistent with each lot
Note: Due to the nature of tissues, some time even house keeping genes show weak signal or no signal in some tissues. So GAPDH and b-actin cannot be guaranteed to show strong signals at the same time.
Storage and Stability:Shipping: Dry IceStorage: -70°C for Total RNA and long term protein, -20°C for short term protein, 4°C for genomic DNAShelf Life: Half a year from the date of receipt under proper storage condition
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
