ZAP70 is a non-receptor protein tyrosine kinase (part of the Syk/ZAP70 family) that is involved in signaling by the T-cell antigen receptor (TCR). Ligation of the TCR/CD3 receptor in Jurkat T-cells induces phosphoprotein complexes that contain ZAP70 (1). TCR zeta chains are initially phosphorylated by p56Lck that lead to the recruitment of ZAP70 via its SH2 domain. ZAP70 in turn phosphorylates other proteins in the TCR-phosphoprotein complex. One of the natural substrates for ZAP70 is the zeta-chain dimer of the TCR/CD3 complex (2).
Source: Recombinant full-length human ZAP70 was expressed by baculovirus in Sf 9 insect cells using an N-terminal GST tag. The gene accession number is NM_001079.
Molecular Mass: The approximate molecular weight is 96 kD (see Figure 1 below).
Activity: The specific activity of ZAP70 was determined to be 113 nmol/min/mg using a Poly (Glu:Tyr, 4:1) synthetic peptide substrate (see activity assay protocol).
Storage: This product is stable at ≤ -70° C for at least 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
仕様
Size：10ug
Source Antigen：Recombinant, Baculovirus in Sf9 insect cells
Grade：Highly Purified
Purity：≥ 95% by densitometry
Form：Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
