IBSP (integrin-binding sialoprotein; also BSP or bone sialoprotein (II)) is a 55-75kD, secreted, variably glycosylated, monomeric noncollagenous member of the SIBLING family of extracellular matrix (ECM) proteins. It is principally associated with the early stages of bone mineralization. BSP is synthesized as a 317 amino acid (aa) precursor that contains a 16aa signal sequence and a 301aa mature region. BSP is highly glycosylated, sulfated, and phosphorylated. Phosphorylation may impact HAp growth, while carbohydrate may regulate cell adhesion. Mature human BSP is 70%, 72%, 78%, and 72% aa identical to porcine, rat, canine, and mouse BSP, respectively. BSP is synthesized by megakaryocytes/platelets, osteoblasts, osteocytes, odontoblasts, osteoclasts, and bone marrow stromal cells.
Recombinant protein corresponding to Phe17-Gln317 with a C-Terminal 10-His Tag from human human BSP, expressed in CHO cells (AAC95490).
Molecular Mass:Recombinant human BSP migrates as an ~75-90kD protein in SDS-PAGE under reducing conditions.
Activity:Measured by the ability of the immobilized protein to support the adhesion of MCF-7 cells. When 5x10e4 MCF-7 cells/well are added to recombinant human IBSP-coated plates (3ug/ml with 100ul/well), ~60-80% will adhere after 30 minutes at 37°C. Optimal concentration depends on cell type.
Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, CHO cells
Grade：Purified
Purity：≥90%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: ≤1EU/ug (LAL).
Form：Supplied as a lyophilized powder from 20mM MES, 0.5M sodium chloride, pH 6.5. Reconstitute with 500ul sterile PBS.
