SGK1 is a member of the serum- and glucocorticoid-induced protein kinase that is activated in vitro by 3-phosphoinositide- dependent protein kinase-1 (PDK-1) and in vivo in response to signals that activate phosphatidylinositol (PI) 3-kinase (1). SGK1 mRNA is expressed in all tissues and the level of SGK1 mRNA is increased by stimulation with serum or dexamethasone. SGK1 promotes cell survival by phosphorylating and inactivating FKHRL1 (2). SGK and Akt display differences with respect to the efficacy with which they phosphorylate the three regulatory sites on FKHRL1.
Source: Recombinant human SGK1 (amino acids 60-431) was expressed by baculovirus in Sf 9 insect cells using an N-terminal GST tag. The gene accession number is NM_005627.
Molecular Mass: The approximate molecular weight is 73kD
Activity: The specific activity of SGK1 was determined to be 117 nmol/min/mg using a synthetic peptide substrate (RPRAATF) (see activity assay protocol).
Storage and Stability: This product is stable at ≤ -70° C for at least 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
仕様
Size：10ug
Source Antigen：Recombinant, Baculovirus in Sf9 insect cells
Grade：Highly Purified
Purity：≥ 95% by densitometry
Form：Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
