NEK2 is closely related in its catalytic domain to the Serine/Threonine protein kinase NIMA of Aspergillus nidulans that is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2. Like NIMA, the NEK2 protein is almost undetectable during G1 but accumulates progressively throughout S, reaching maximal levels in late G2 (1). NEK2 is shown to be expressed most abundantly in the testis of the adult tissues examined being localized to the nucleus (2).
Source: Recombinant full-length human NEK2 was expressed by baculovirus in Sf 9 insect cells using an N-terminal GST tag. The gene accession number is NM_002497.
Molecular Mass: The approximate molecular weight is 76 kD (see Figure 1 below).
Activity: The specific activity of NEK2 was determined to be 288 nmol/min/mg using a myelin basic protein (MBP) substrate (see activity assay protocol).
Storage and Stability: This product is stable at ≤ -70° C for at least 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
仕様
Size：10ug
Source Antigen：Recombinant, Baculovirus in Sf9 insect cells
Grade：Highly Purified
Purity：≥ 90% by densitometry
Form：Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
