IL-22 receptor, also known as IL-22Rα1 and CRF2-9, is an approximately 65kD transmembrane glycoprotein in the type II cytokine receptor family (CRF). IL-22R contains a 211 aa extracellular domain (ECD) with two fibronectin type III repeats, and a 323 aa cytoplasmic domain. IL-22R associates with either IL-10Rβ or IL-20Rβ to form receptor complexes with distinct ligand selectivities. IL-10Rβ is a shared subunit of the IL-10, -22, -26, -28, and -29 receptors, while IL-20Rβ is a shared subunit of the IL-19, -20, -22R and -24 receptors. 1 IL-22R/IL-10 Rβ is an IL-22 responsive receptor 2, 3 , and IL-22R/IL-20Rβ is an IL-20 or IL-24 responsive receptor. 4, 5 IL-22R contains cytoplasmic motifs for interactions with signal transduction molecules, but formation of ternary complexes with IL-10Rβ or IL-20Rβ and the respective ligands is required for signal transduction. 2, 6 IL-22BP functions as a competitive antagonist by binding IL-22 and preventing its association with IL-22R. 7, 9 Even though it is a receptor for interleukins, IL-22R is not expressed on hematopoietic cells. 6, 10, 11 Instead, IL-22R expression is restricted to epithelial and stromal cells. 6, 10-13 IL-22R signaling promotes innate immune responses and wound healing at sites of infection and inflammation. This includes upregulation of antimicrobial, acute phase, proinflammatory, and extracellular matrix proteins as well as proteases. 3, 11, 13, 14 IL-22R signaling also promotes downregulation of proteins involved in keratinocyte differentiation. 3, 14 Within the ECD, human IL-22R shares 78%, 76%, and 83% aa sequence identity with mouse, rat, and canine IL-22R, respectively. It shares 22%-25% aa sequence identity with the ECDs of other class II receptors IL-10R, IL-20R, and IL-28R.
Recombinant protein corresponding to Pro18-Thr228 with a C-Terminal 6-His-Tag from human IL-22R, expressed in NS0 cells. Accession: NP_067081.
SDS-PAGE:34-38kD under reducing conditions
Activity:Measured by its ability to inhibit rhIL-22-induced IL-10 secretion by COLO 205 human colorectal adenocarcinoma cells. The ED50 for this effect is typically 1-4ug/ml in the presence of 1ng/mL of rhIL-22.
Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, NS0 cells
Grade：Highly Purified
Purity：≥95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: ≤0.1EU/ug (LAL)
Form：Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitute with 500ul sterile PBS.
