Human Ficolin 2 is a member of the ficolin family of secreted pattern recognition proteins that participate in the lectin complement activation pathway. Ficolin 2 is expressed in the liver and released into the circulation. The 35-40kD, 313 amino acid (aa) human Ficolin-2 contains a 25 aa signal sequence, an N-terminal collagen domain and a C-terminal fibrinogen-like domain that includes a calcium binding site and two potential N-glycosylation sites. The collagen domain mediates trimer formation. Larger homo-multimers are formed by disulfide links at the N-terminus, the most prominent of which is a 12 subunit oligomer. Ficolin 2 binds microbial ligands that contain acetylated compounds. Notably, this includes N-acetyl glucosamine in compounds such as lipoteichoic acid in gram-positive bacteria. It also binds fungal 1,3-beta-D-glucan. Pathogen recognition by Ficolin-2 initiates an immune response that involves calcium-dependent interaction of Ficolin 2 with the MBL-associated serine protease (MASP) complex. This complex cleaves C4 to activate the complement pathway. In a secondary role, Ficolin 2 is known to bind late apoptotic and necrotic cells, probably through the recognition of exposed DNA. This also activates the complement cascade that assists in clearance of cells. Mature human Ficolin-2 shares 70%, 72%, 76% and 78% aa identity with mouse, rat, cow and pig Ficolin 2, respectively. It shares 84% and 52% aa identity with human ficolin 1 and ficolin 3, respectively. Single nucleotide polymorphisms are common in human Ficolin 2. Some affect serum concentration, while others can increase or decrease ligand binding.
Recombinant protein corresponding to Leu26-Ala313 from human Ficolin 2, fused to a C-terminal 10X His-Tag, expressed in NSO cells (Q15485).
Activity:Measured by its ability to bind biotinylated N-Acetylglucosamine-Polyacrylamide in a functional ELISA type binding assay. When Recombinant Human Ficolin-2 is immobilized at 2ug/ml, Recombinant Human MBL binds with an ED50 of 2.5-15ug/ml.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, NSO cells
Grade：Highly Purified
Purity：≥95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: ≤0.01EU/ug (LAL).
Form：Supplied as a lyophilized powder from PBS. Reconstitute with 500ul sterile PBS.
