The mature recombinant human BMP-10, generated by the proteolytic removal of the signal peptide and the propeptide, is a disulfide-linked homodimeric protein. Based on N-terminal sequencing, the mature recombinant human BMP-10 starts at Asn 317. The monomer has a calculated molecular mass of 12.2kD and migrates as an approximately 12.2kD protein in SDS-PAGE under reducing conditions.
Source:Recombinant corresponding to Asn317-Arg424 from human BMP10 expressed in CHO cells (O95393).
Activity:Measured by its ability to induce alkaline phosphatase synthesis by MC3T3E1 osteoblastic cells (Mazerbourg, S. et al., 2005, J. Biol. Chem. 280:32122). The ED50 for this effect is typically 15-60ng/mL.
Reconstitution:It is recommended that sterile 4mM HCI containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10ug/mL.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 4mM HCI, with ≥0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：25ug
Source Antigen：Recombinant, Chinese Hamster Ovary (CHO) cells
Grade：Highly Purified
Purity：~97%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: ≤1EU/ug (LAL).
Form：Supplied as a lyophilized powder from 30% Acetonitrile, 0.1% TFA, 1.25mg BSA. Reconstitute to 10ug/ml in sterile 4mM HCI, with ≥0.1% HSA or BSA.
