Retinal progenitor cells (RPCs) retain the ability to generate different retinal cell types. RPCs appear to be retained in a progenitor state by the action of Notch-Delta signaling and downstream effectors of Notch, like the bHLH transcription factor Hes1. The most uncommitted RPCs (retinal stem cells) in the ciliary margin of the Xenopus retina were shown to coexpressthe transcription factors Pax6, RX1, and Six3 prior to the activation of neurogenic genes (Notch, Delta, bHLH) and the subsequent steps toward retinal neurogenesis. Pax6 is required for the multipotent state of retinal progenitor C. Upon Pax 6 inactivation, the potential of RPCs becomes entirely restricted to only one of the cell fates normally available to RPCs resulting in the exclusive generation of amacrine interneurons. Pax6 has been found to directly control the transcriptional activation of retinogenic bHLH factors that bias subsets of RPCs towards different retinal cell fates, thereby mediating the full retinogenic potential of RPCs.
Applications:Suitable for use in Antibody blocking. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Form：Supplied as a lyophilized powder. Reconstitute to 1mg/ml in ddH2O.
