85-1533-55　[2-(Trimethylammonium)ethyl] Methanethiosulfonate Bromide (MTSET Bromide) 250mg　CAS No:91774-25-3　T8488

特徴

• Reacts specifically and rapidly with thiols to form mixed disulfides. Used to probe the structures of the ACh receptor channel of the GABAA receptor channel and of lactose permease.
• At pH 7.5 and room temperature, MTSEA hydrolyzes with a half-life of about 15 minutes, MTSET hydrolyzes with a half-life of 10 minutes, and MTSES hydrolyzes with a half-life of 20 minutes. Routinely, one can use 2.5mM MTSEA, 1mM MTSET, or 10mM MTSES, applied for 1 to 5 minutes. (MTSET is 2.5 times as reactive with small sulfhydryl compounds as is MTSEA, and 10 times as reactive as MTSES). (Stauffer and Karlin, 1994; Karlin, personal communication).
• For application to Xenopus Oocytes, dissolve the compounds in 115mM NaCl, 2.5mM KCl, 1.8mM MgCl2, 10mM Hepes, pH 7.5 with NaOH. MTS reagents decompose in buffer very quickly
• DMSO is a good solvent for the MTS reagents which are not water soluble (i.e. the non-charged MTS reagents).
• Synonyms:N,N,N-Trimethyl-2-[(methylsulfonyl)thio]-ethanaminium Bromide; MTSET;(2-Mercaptoethyl)trimethylammonium Bromide Methanesulfonate; Methanethiosulfonate Ethyltrimethylammonium Bromide
• Molecular Formula:C6H16BrNO2S2
• Molecular Weight:278.23
• Purity:≥95%
• Appearance:White solid
• Solubility:Methanol (Slightly), Water (Slightly)
• Method for Determining Identity:Proton NMR Spectroscopic and Mass Spectrometric Analysis
• TLC Conditions:C18; Water: Isppropanol: Acetic Acid=6:4:1; Visualized with AMCS and KMnO4; Single Spot, Rf=0.65
• Melting Point:151-158°C
• Bromide:As Reported
• Elemental Analysis:Calculated: %C: 25.90, %H: 5.80, %N: 5.03
• Storage and Stability:May be stored at RT for short-term only. Long-term storage is recommended at -20°C. Stable for 6 months after receipt under inert atmosphere.. For maximum recovery of product, centrifuge the original vial prior to removing the cap.