Method for Determining Identity:Proton NMR (CDCl3) Spectroscopic and Mass Spectrometric Analysis
TLC Conditions:SiO2; Ethyl Acetate=100%; Visualized with AMCS and KMnO4; Single spot, Rf=0.3.
Storage and Stability:May be stored at RT for short-term only. Long-term storage is recommended at 4°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Store under inert conditions. Moisture sensitive.
At pH 7.5 and room temperature, MTSEA hydrolyzes with a half-life of about 15 minutes, MTSET hydrolyzes with a half-life of 10 minutes, and MTSES hydrolyzes with a half-life of 20 minutes. Routinely, one can use 2.5mM MTSEA, 1mM MTSET, or 10mM MTSES, applied for 1 to 5 minutes. (MTSET is 2.5 times as reactive with small sulfhydryl compounds as is MTSEA, and 10 times as reactive as MTSES). (Stauffer and Karlin, 1994; Karlin, personal communication).
For application to Xenopus Oocytes, dissolve the compounds in 115mM NaCl, 2.5mM KCl, 1.8mM MgCl2, 10mM Hepes, pH 7.5 with NaOH. MTS reagents decompose in buffer very quickly
DMSO is a good solvent for the MTS reagents which are not water soluble (i.e. the non-charged MTS reagents).
Toxicity and Hazards:Due to the potential danger associated with the use of some of United States Biological biochemicals, all products should be handled by qualified personnel only, trained in laboratory procedures, and familiar with potential hazards. Information is not available on the possible hazards of many experimental compounds. The absence of a warning must not be interpreted as indication of safety. The ultimate responsibility for the proper handling of any and all chemicals lies with the user. All goods sold by United States Biological carry the condition that liability for any injury, loss or damage whatsoever to persons or real property resulting from the handling of the said goods lies with the user. Acceptance of our goods binds the customer to these terms.
