An active metabolite of Sulindac. Inhibits cyclooxygenase, but induces apoptosis by a cyclooxygenase-independent mechanism. Inhibition of Ras activation of Ref-1. Impairs nucleotide exchange on Ras by CDC25 and accelerates Ras hydrolysis of GTP by p120GAP.
Method for Determining Identity:Proton NMR Spectroscopic Analysis
TLC Conditions:Dichloromethane: Methanol; 9:1; Visualized with UV and AMCS; Single spot; Rf = 0.56.
Storage and Stability:May be stored at RT for short-term only. Long-term storage is recommended at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
