Monobromobimane readily reacts with low molecular weight thiols. Bimanes are useful for detecting the distribution of protein thiols in cells before and after chemical reduction of disulfides.
Fluorescence:max. Abs. 398nm; e x 10-3: 5.0
Melting Point:158-160°C
Solubility:Dichloromethane, DMSO
Method for Determining Identity:Proton NMR Spectroscopic Analysis
TLC Conditions:SiO2:Dichloromethane: Methanol: 9.5: 0.5. Visualized with UV; Single spot; Rf=0.50.
Storage and Stability:May be stored at RT for short-term only. Long-term storage is recommended at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
