Production of ceramide occurs upon hydrolysis of sphingomyelin by a specific isoform of phospholipase C, appropriately named sphingomyelinase.
Melting Point:83-85˚C
Solubility:Chloroform
Method for Determining Identity: Proton NMR Analysis and Mass Spectrometric Analysis
TLC Conditions:SiO2; Dichloromethane : Methanol : Ammonium Hydroxide (9:1:0.2). Visualized with AMCS. Single spot (Rf=0.55).
Storage and Stability:May be stored at RT for short-term only. Long-term storage is recommended at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
