A protected 5(6)-Carboxyfluorescein in which the hydroxyl groups are acylated with isobutyric acid. The protection prevents self-condensation during carboxyl activation andavoids the formation of fluorophore-oligomer containg by-products associated with the use of unprotected FAM.
Method for Determining Identity:Proton NMR Spectroscopic and Mass Spectrometric Analysis
TLC Conditions:SiO2; Dichloromethane: Methanol; 9:1; Visualized with UV and AMCS; Rf=0.35
Ethly Acetate Content:~2%
Atmoshere:Inert Gas
Storage and Stability:May be stored at RT for short-term only. Long-term storage is recommended at -20°C for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Store Under inert atmosphere.
