Method for Determining Identity:Proton NMR (CDCL3) and Mass Spectrometric Analysis
TLC Conditions:SiO2; Dichloromethane : Methanol: Ammonium Hydroxide= 9:1: 0.05; Visualized with AMCS, Single Spot, Rf=0.45
Atmosphere:Inert Gas
Storage and Stability:May be stored at RT for short-term only. Long-term storage is recommended at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Hygroscopic. Store under inert atmosphere.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Toxicity and Hazards: All products should be handled by qualified personnel only, trained in laboratory procedures.
Storage and Handling Notes:Some methanethiosulfonates are hygroscopic and all hydrolyze in water, over a period of time, particularly in the presence of nucleophiles. They should be stored in a desiccator at minus 20°C and warmed up to room temperature before opening the vial. For maximum results, solutions should be made up immediately prior to use even though solutions in distilled water appear to be stable for hours at 4°C.
Application Notes:At pH 7.5 and room temperature, MTSEA hydrolyzes with a half-life of about 15 minutes, MTSET hydrolyzes with a half-life of 10 minutes, and MTSES hydrolyzes with a half-life of 20 minutes. Routinely, one can use 2.5mM MTSEA, 1mM MTSET, or 10mM MTSES, applied for 1 to 5 minutes. (MTSET is 2.5 times as reactive with small sulfhydryl compounds as is MTSEA, and 10 times as reactive as MTSES). (Stauffer and Karlin, 1994; Karlin, personal communication).
For application to Xenopus Oocytes, dissolve the compounds in 115mM NaCl, 2.5mM KCl, 1.8mM MgCl2, 10mM Hepes, pH 7.5 with NaOH. MTS reagents decompose in buffer very quickly.
Solubility Note:DMSO is a good solvent for the MTS reagents which are not water soluble (i.e. the non-charged MTS reagents).
