A thiol specific fluorescent probe useful for ion channel and protein topology research. The recently available MTS-fluorophores may find application in the real-time monitoring of conformational changes, since fluorophores coupled to introduced cysteines can change their fluorescence during a conformational change. Fluorescence lifetime may also yield information regarding distances and molecular motion in a protein molecule.Fluorescence: max. Abs. 492nm; max. Em. 515nm; e x 10-3: 75
Synonyms:MTSEA-Fluorescein Trifluoroacetic Acid Salt
Molecular Formula:C₂₆H₂₁F₃N₂O₁₀S₂
Molecular Weight:642.58
Purity:≥95%
Appearance:Yellow to orange solid
Melting Point:>130°C (dec.)
Solubility:DMSO (Slightly)
Atmosphere:Inert
Method for Determining Identity:Proton NMR and Mass Spectrometric Analysis
TLC Conditions:SiO2; Dichloromethane: Methanol=9:1; Visualized with UV, KMnO4 and Naked Eye; Single spot, Rf=0.50
Storage and Stability:May be stored at RT for short-term only. Long-term storage is recommended at -20°C. Stable for 6 months after receipt. Hygroscopic. Store under inert atmosphere.