Human Lymphotoxin beta receptor (LTbR, TNFSF3) is a member of the TNF receptor superfamily with similarilty to CD120a(TNFR1) and CD120b(TNFR2). It is a expressed mainly on non lymphoid tissues(1) and appears to have an important role in secondary lymphoid organ development(5). Suppression of LTbR signaling can alleviate autoimmunity(2) or exasperate mycobacterial infection(6). Its ligands include Ltab 2 and LIGHT. In mice, LTbR signaling is important for development and function of HEV(2), Dendritic cells(3), and Mast cells(4).
Molecular Structure:A soluble fusion protein consisting of 10 residual amino acids from murine CD8 alpha leader sequence (KPQAPELRGS), followed by the mature extracellular (192aa) domain of human LTbR (SQPQAVP….PLEPLPP) fused to (233aa) murine IgG2a Fc (EPRGPTI….FSRTPGK). Predicted non-glycosylated molecular weight of 48.9kD. LTbR-muIg identity was confirmed by N-terminal sequencing (KPQAP) and amino acid analysis.
Performance:Recombinant human LTbR-muIg fusion protein binds to recombinant human LIGHT in EIA. LTbR-muIg was detectable in EIA (at 50-500 ng/ml) using recombinant human LIGHT coated plate for capture and GAM/HRP as a detector.
Applications:Suitable for use in ELISA. Other applications not tested.
Storage and Stability:May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：25ug
Source Antigen：CHO cells
Grade：Purified
Purity：Purified by affinity and size exclusion chromatography.
Form：Supplied as liquid in 50mM sodium phosphate pH 7.5, 100mM potassium chloride, 150mM sodium chloride and 0.5mg/ml gentamicin sulfate.
