Human CD134 (OX40) (ACT35) is an activation-associated antigen which is predominantly expressed on activated CD4 positive cells. CD134 antigen is a member of the tumor necrosis factor (TNF) receptor family of molecules and may be involved with regulating T cell-dependent B cell proliferation and differentiation (2).
The CD134 costimulatory pathway seems to be more effective for costimulation of CD4+ helper T cells than for CD8+ effector T cells (5). Blockade of this interaction in mouse abrogated immunological effects in several models of immflamation and rejection (6,7,8,9). CD134-muIg fusion protein binds to CD134L on Human Umbilical Cord Endothelial Cells (HUVEC).
Human CD134(OX40)-muIg Fusion Protein*
Molecular Structure:A soluble molecule consisting of the extracellular (185 aa) domain of human CD134 fused to the murine IgG2a Fc (232 aa). Predicted monomeric non-glysylated weight is 45.6kD. Fusion construct is dimeric and runs about 105kD in non-reduced SDS-PAGE.
Applications:Suitable for use in ELISA, Flow Cytometry. CD134-muIg fusion protein blocks binding of anti-human CD134 to human tumor cells. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：25ug
Source Antigen：CHO cells
Grade：Purified
Purity：Purified by size exclusion chromatography.
Form：Supplied as a liquid in 50mM Sodium Phosphate pH 7.6, 100mM sodium chloride, 0.5mg/ml Gentamicin Sulfate.
