CD80, also known as BB1/B7-1, is a 60kD type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed on activated B cells, T cells, monocytes and dendritic cells. It is a ligand of the two T cell costimulatory molecules, CD28 and CD152 (CTLA-4). The interaction of CD80 (or CD86) with CD28 provides a costimulatory signal for T cells activated through the CD3/TCR complex.
Molecular Structure:A soluble dimeric fusion protein consisting of the extracellular (173aa) domain of human CD80 fused to murine IgG2a Fc + hinge (232 aa), with a predicted monomeric molecular weight of 51.3kD.
Functional Application:CD80-muIg fusion protein blocks binding of anti-human CD80 to human tumor cells.
Applications:Suitable for use in ELISA, Flow cytometry and Antibody Blocking. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：25ug
Source Antigen：CHO cells
Grade：Purified
Purity：Purified from tissue culture supernatant of CHO transfectants.
Form：Supplied as a liquid in 50mM sodium phosphate pH 7.6, 100mM sodium chloride, 0.5mg/ml gentamicin sulfate.
