Human CD54 (ICAM-1) mediates cell adhesion by binding to the integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). CD54 expression on resting peripheral blood leukocytes is weak but is upregulated on activated T and B lymphocytes and on monocytes.
Molecular Structure: A soluble dimeric fusion protein consisting of the extracellular (451aa) domain of human CD54 (including signal peptide) fused to murine IgG2a Fc (232aa). Predicted monomeric molecular weight of mature construct 76.1kD (amino acid composition only).
Transfectant Cell Line: CHO
Human CD54(ICAM-1)-muIg Fusion Protein
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer
仕様
Size:25ug
Source Antigen:CHO cells
Grade:Affinity Purified
Purity:Purified by affinity chromatography.
Form:Supplied as a liquid in FBS, 50mM Sodium Phosphate pH 7.5, 100mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin Sulfate.