Human CD40 is a member of the tumor necrosis factor (TNF) receptor family and is present on all B cells except plasma cells. CD40 is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 plays an important role in B cell activation and the interaction with its ligand CD154 (CD40 Ligand) is essential for isotype switching (1). CD40 muIg fusion protein is recognized by anti-human CD40 antibody and binds to human CD154. This binding can be blocked by anti-CD154 antibody.
Molecular Structure: A soluble fusion protein consisting of the extracellular (173aa) domain of human CD40 fused to murine IgG2a Fc (233aa).
Functional Application: CD40 Human, IgG2a Fc Mouse, Fusion Protein blocks binding of anti-human CD40 to Raji human tumor cells.
Flow Cytometry Protocol:1. Jurkat-4G (a CD154 positive subclone of Jurkat)cells were stimulated 6 hours in the presence of PMA and Ionomycin (to increase CD154 expression). 2. Five x 10e5 cells per tube were incubated 45 minutes on ice with 80ul of C2392-02KX at 5ug/ml. 3.Cells were washed twice and incubated with IgG (FITC) Goat anti-Mouse
4. Cells were washed three times, fixed and analyzed using a BD FACS Calibur. 5. Cells stained positive with a mean shift of 0.22 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration
Applications:Suitable for use in ELISA, Flow cytometry. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer
仕様
Size：25ug
Source Antigen：CHO cells
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in FBS, 50mM Sodium Phosphate pH 7.5, 100mM Potassium Chloride, 150mM NaCl.
